Elispot 5.0 (from build 8263) - new (additional) variant of QC function - impruvements in presentation data for multi-image plates - new revision of export scripts (from build 7876) - improvements in support Sony cameras - added support Allied Dolphin cameras - improvements in calculation of activity in case of manually added spots (from build 7804) - optimisations and improvements in plate history - plate layout improvements for case two-image reading mode (from build 7722) - new user specific toolbar (from build 7647) - new hardware variants suppot: Stingray cameras - excel export: new combinations for presentations of results and impruvements in previously exist functions - fixed error in case of view activity for invert conting case with TNTC (in plate view number of spots was not visible) - QP/SP movements optimisation: added elements of self diagnostics - unification of robot functions for compatibility with IFL Robot (from build 7295) - impruvements in work of user management - function for saving additional(intermedia) images in two-image reading mode - new function/option "View result of analyse" in plate view - new function that calculate distribution of intensities for double stained spots (from build 7124) - software has some changes in interface for options - impruvements in work of IFL (iSpot) modules - additional possibilities in data export (Excel) - IFL parameters (camera settings, filters, reading mode) now are users specific - in users management system added possibility to control users groups - minor impruvements in CountAID - adjustale toolbar in plate view Main differences with previous version(s) (4.0): (from build 6450) - software support IFL readers hardware. - software has some additional functions and options (special acquisition and viewing modes, data presentations) that relative with possibilities IFL hardware. - new revision of CountAID (learning) function. - corrected some compatibility problems with old readers hardware. (from build 5675) - added new type of motor control (TMCM). - added support for "optical cube" hardware. (from build 5527) - fixed problem in rule compiler in layout for case: group criteria on + positive control + well(s) TNTC (or same for all Wells Saturation > limit). (from build 5396) - fixed problem with eject position for new readers' cases. - support of XL type readers. - improvements in module of IFL type readers for camera initialisation. - some optimisations in software options. (from build 5098) - fixed mistake situation after activation mode "view outlines". This problem was observable only in builds 4980-5092. (from build 5092) - some optimization in work with memory allocation (reduced amount of requred memore on 20%) (from build 5057) - this is special build that compatible with ViruSpot hardware (from build 5018) - Fixed mistake if IAVI data set used for data export in txt format: number spots was use without thresholds. (it was observable in previous versions /build 4001-5017/ only if thresholds activated and only for IAVI data set) (from build 4990) - CountAID improvements - New global modes of work (FDA,Reasearch,Diagnostic) - New services (bacukp,calibration,QC reminders) - TNTC (from build 4667) - Reorganization of Layout functions (completely compatible with previous version) - New function for viewing layout components (from build 4313) - QC function calculations improved - Additional options for "2-color assay" function (from build 4174) - RCA function improved - Interface improvements in creating user Layouts - Possibility to export layout data in text format - AOI as separate option in user profile Fixed problem(s): - Password length can be more than 7 Features: (from build 4031) - RCA (Read-Count-Analyse) function - Two-color optimised routine - CountAID function - Enhanced CompareWells function - Rule compiler - Possibility to create user Layouts - Importing count settings from previous EliSpot versions - Supporting new EliSpot hardware with automated camera adjustment Elispot 3.5 Main differences with previous version(s) (3.4): Features: (from build 2245) - Optimisation in interface of the program: Properties (Options) dialogs, Plate View, Well View. - Advanced comments (flags, specifications). - Flexible adjusting for interface, export. Fixed errors (problems): In Build 2550: For case of DBS camera type fixed problem with stage calibration. In Build 2344: For Algorithm 2.6-2.9 fixed situation "An exception occured while counting well ..." . Elispot 3.4 Main differences with previous version(s) (3.2.3 ): Features: (from build 1336) - Adjustible scale(ruler) in stage calibration and scale based size of pixel. - Function for resetting of AOI. - Some optimisation of stage calibration for HR systems. (from build 1196) - It is possible for analysis distribution of "Cytokines" (ranges: LOW, LOW-MEDIUM, MEDIUM-HIGH, HIGH). Results available in Well view diagram or in in Excel data. Ranges can be adjustable. - "Overall Cytokines" calculated on base absolute intensities of spots (NOT relative to background like was before). - Possible view of Plate with as Number of spots as with Overall Cytokines. - New more flexible Re-count Directory function. - In Well view added parameter "Overall Cytokine". This parameter will be useful to routine diagnostic. - Enhanced working with memory. Stability of algorithms increased. Fixed errors: (from build 1328) - Fixed errors for case when was no update state of count settings in dialog bar in Well View. (from build 1196) - Fixed Error with long name and(/or) path for plate document (case when name longe than 64 simbols). - Fixed bug with "Overall Area" in well view. Before it was counted for all spots, now only for the spots that are in range. EliSpot 3.2.3 ----------------------- Main differences with previous version(s) (3.2.2): Fixed errors: - Select AID default camera setting for new cameras now work as from properties dialog as from main menu. (Before function was not always working in main menu) - Toolbar is correct after closing of plate if few plates are open. (Some of buttons before was not available). - View Help available as from Plate view as from well view as by pressing F1. - "Eraser" can delete every spot in well view. Before it has sometimes problem if program detect few very closely located spots. - program not crush in case of using "Eraser" or "Add spot" (before it was observable with some specific count settings) New features: - In Count Settings Dialog expended options to select active algorithms for counting. Now possible: 2.6-2.9, 3.0-3.1.x, 3.2.x (at the moment last means 3.2.2 and 3.2.3). - Context help in Calibration Dialog (If cursor arrows on dialog control about second will appear context tip or help information). - Help View available if on PC installed any version of Adobe Acrobat Reader (Even not exist yet). Before it was possible to have it only with Ver. 4.0 or 5.0. - For AID High Resolution Reader type available new option - counting with precision of AID Standard Resolution system. - optimization in loading of plate documents. - some small changes in interface. EliSpot 3.2.2 ----------------------- Main differences with previous version(s) (3.2.1): - Program has modified (!!!) count algorithm in part parameters that determine sensitivity to detect objects. (Now program can detect more small and weak spots). - Program has optimised default parameters for cameras (IEEE1394 cameras following DCAM- Standard and compatible with OCHILynx-1394-Hostconroller driver). - Fixed some small errors in manipulations with Diagram Dialog and in access to camera settings. - Maximal number of count setting for one user profile now is 100. EliSpot 3.2.1 ----------------------- Main differences with previous version(s) (3.1, 3.1.*): - Program has special control bar for scrolling of the wells in plate and manipulation with threshold values without recounting. - Program support new camera driver and hardware (IEEE1394 cameras following DCAM- Standard and compatible with OCHILynx-1394-Hostconroller). - Program has optimization in improvements in Plate HISTORY. - Program has new possibilities in User Managements. EliSpot 3.1 ----------------------- Main differences with previous version (3.0 rev.3): - Program has new count settings and new count dialog. Now it is possible to determine MIN and (/or) MAX values for cutoff. - Program support HISTORY for every plate. (It is possible to get information about Reader Unit ID, Camera, Count Settings and etc.) - Program support additinal new VBS scripts with Excel template (EliStat). - Option "Interpretation Level" that allow detecting wells that are over development now is not global and it is a feature of every count setting. - Program has function "Count Directory" for automatically recounting of big amount of data. - Program automaticaly determines(plug-and-play) connection with QuadPack motor control. - Program has additional possibilities in User Management System. (User Profiles now has more options) - Program support as Sony DFW-V500 cameras as Sony DFW-SX900 with original Sony driver. (For this adjusted calibration dialog view, modified auto center function, and calibration calculation functions) - Program detects type of the camera with Sony driver automatically - Some addings in stage calibrations - In program fixed few errors. ===================================