Primer Premier 4.04

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    PRIMER PREMIER is one of the most comprehensive primer design tools available to design optimal primers for PCR, sequencing and hybridization quickly. Primers can be designed automatically or manually. With the automatic search, the program gradually relaxes the stringency of the search until the best possible primers available within the target sequence are found. The primers are located to optimize for Tm, GC%, GC clamping, 3` end stability, and to minimize hairpins and dimers. For experimental designs requiring a finer degree of control, the manual search allows exact primer criteria to be set and all primers meeting the criteria are located. The resultant primers are ranked both individually and paired based on their priming efficiency. The selected primers or pairs can be ordered easily by printing the synthesis order form provided.

    Homepage: http://www.premierbiosoft.com/