Rotor-Gene Version History

Features have been introduced in order to allow this version and RG6000 Series Software to coexist on the same system.

Occasionally, clicking on the cancel button of the sample editor's colour dialog would cause an error message to appear. This bug has now been fixed.

Saving a run file in version 5.0 format would previously not correctly produce the file. This has now been resolved.

Certain profiles can cause display issues in the temperature graph making the temperature appear much higher than the actual value. This has been fixed.

If the sample editor was open and the first data acquisition occurs while the editor is still open, and error message will appear when the user closes the window. This has now been fixed.

An unneccessary error message was being raised when a PC has been on after 24 days without rebooting. This message has been removed.

Code from the upcoming 7.0 release was accidentally introduced into 6.0 causing a irregular jump in the temperature graph.

Internally REX files are essentially still a text file format. Because of this email servers often try to optimise the content of REX files which can cause corrupted temperature values when the software reinterprets the mangled data. The software now has improvements to prevent this from happening.

Improvements have been made to minimise library conflicts with other software packages that may be installed on the same PC.

More scientific formats have been provided for the given concentration, allowing customers to use higher precision numbers with scientific notation.

Rotor-Gene run files were being saved with the wrong user permissions causing problems for customers who share their data over a network. This issue has been resolved.

Corrected bug in reporting mechanism which would occur on certain Windows locales.

Analysis options are no longer restricted when running the Quenched FRET template from the quick start wizard.

When running the software in virtual mode the temperature appears to cool immediately. This has been fixed.

Due to a bug in the Windows operating system, if a PC has not been rebooted for more than 24 days this can trigger an error in the Rotor-Gene software. The software has been modified to cope with this glitch in Windows.

The handling of USB Converters has been improved, minor start up issues with converters are now handled gracefully.

Auto gain settings in Sybr Green template have been updated to improve the fluorescence range. This overcomes problems where a minimum range of 0 Fl. causes extremely low gain to be selected.

Fixes a discrepancy between exported results and report results in Relative Quantitation analyses.

Rotors with numeric tube labelling are now fully supported, numeric tube labelling is now used as default.

Simplified recovery from loss of factory calibration settings.

Fixes an error that appears when opening reports after installing the Corbett Robotics software. This was due to a library conflict with the Corbett Robotics software.

Sample line pattern now saves and loads correctly.

Fix display of windows on non-4/3 aspect ratios.

Reports now display correctly on a Windows 98 system, overcoming a previous issue.

Fixed a minor bug where is was possible to leave the Advanced Wizard without specifying concentrations for all standards.

Empty columns now do not appear in the exported Excel spreadsheet when using the "Export to Excel" function from the result grid.

Errors relating to the import of older sample import files have been resolved.

The COM port selector is now visible in the Welcome screen, allowing for the selection of a COM port other than the previous default of COM1.

The Concentration Measurement profile may now be modified, making it more useful for other applications of this analysis such as using the RG as a fluorometer.

Rotor-Gene Run Archives are now saved correctly.

An timeout issue which occasionally occured during the auto-find threshold function has now been resolved.

Machine communications errors experienced when changing rotor speed under some circumstances have been resolved.

An incorrect formula was being used by the Delta Delta CT relative quantitation module to calculate relative concentration. This formula has been corrected.

Fix result grid copy to Excel leaving empty columns.

Added Control column to Comparative Quantitation analysis.

Minor changes to captions.

Fix bug where browsing up one level in the template selector would cause the items to be displayed in alphabetical order.

The wizard used to configure the experiment is now displayed in the Experiment Settings, Messages tab.

Reaction Amplification values are now displayed in Comparative Quantitation Report.

The Melt and Quantitation reports now include the analysed sample page.

The ordering of experiment information on reports has been corrected.

The mean and standard deviation values in the melt reports are now displayed in the user locale, rather than with US locale formatting.

More specific error messages are now given in response to Source and Detector Motor Jam conditions.

Fixed communications error displayed when the chamber lid is open when attempting to start or resume a run.

Importing reaction lists from the Corbett Robotics CAS-1200 has been fixed. This affects other users importing sample files generated by version 5 or earlier of the Rotor-Gene software.

Issues with error messages appearing when rapidly starting and stopping the Rotor-Gene on version 6.0.1 have been resolved.

Improved handling of communication errors has been added.

The back button on the Advanced Wizard is now disabled on the first screen.

A warning is now displayed if users attempt to set High Speed steps in the profile.

Report values were being corrupted on non-English locales. This has been corrected.

Experiment notes are now trimmed when saving in 5.0 format to avoid potential issues loading files with long text.

Improvements have been made to the normalisation and statistical algorithms used in both Quantitation and Comparative Quantitation. This will affect Ct values, Takeoff cycles, and calculated concentrations on existing experiments. The changes use improved statistical models to more accurately calculated unknown concentrations. You should revalidate any existing procedure using these formulae before using in a production environment.

To facilitate the use of published materials, the Rotor-Gene software is changing its reported M and B values to match those used by the competitor standard curve arrangement, that is, "CT = log(conc) * m + b"

As a rearrangement of equations, the former M and B values are still available from the standard curve in the "conc=10^(mx + b)" format on the Quantitation window, and in the reports.

Samples can now be grouped on the display by right-clicking on any graph and selecting "Group By Replicates".

Windows Administors can now define access levels for users on their local computer, or on the entire network. This allows giving permission to perform runs or analysis only to certain users. Samples can be locked at the beginning of a run and then only unlocked by an Administrator, enabling you to enact strong security policies in the laboratory.

The Two Standard Curves method is now available in the software in the new Relative Quantitation module.

The Delta Delta CT method is now available in the software in the new Relative Quantitation module.

To aid new users of the system, a quick start wizard has been added, featuring a rotor-centric sample editor. A default experiment name is now generated based on the template filename, the current date and the run number for the day. This reduces the number of keyboard entry required to begin a run.

The result grid statistics now calculate differently, based on the selected column. For calculated concentrations in Quantitation, the mean and standard deviation are calculated to take into account the exponential statistics model, and so give more meaningful measures of variance. These use orders of magnitude for standard deviation, and the geometric mean for the average.

Replacing the CT standard deviation, a more comprehensive replicate framework has been put in place. Samples with identical names are now treated as replicates, and will have confidence intervals generated for estimated concentrations, CT values and other calculated results.

The results of all analysis can be automatically sent to Excel. Extensions exist to allow this data to be sent to LIMS systems.

To facilitate toggling blocks of samples, a Sample Groups feature enables the user to group related samples. These can then be turned on or off by right-clicking on the sample selector. Sample groups will be eventually used to allow grouping for more complex forms of relative quantitation.

It is now possible to create completely separate sample definitions for each acquired channel, using the new sample pages feature. This allows greater flexibility in designing multiplex experiments, and cleaner result tables for simplex reactions running multiple standard curves.

This new analysis technique enables you to read DNA concentrations on the Rotor-Gene. Comprehensive statistics are generated, enabling you to have confidence in your results.

It is now possible to set the rotor speed at each step, to either high, normal or still on the Rotor-Gene 3000. This enables customers to perform nested amplification.

To allow for cycling profiles which vary substantially in the middle, such as those used for Nested amplification, it is now possible to connect different cycling steps together to create one set of data to be quantitated. This feature now effectively enables any conceivable cycling profile to be programmed.

Annealing Analyses are a useful tool in detecting unexpected additional products in amplification reactions, and are added in this version of the software. This is achieved by setting the profile to ramp downwards in temperature, and then performing melt analysis with the vertical scale flipped.

Easier toggling of options when displaying two quantitation analyses side-by-side with the drop-down menus available for all the hidden toolbar buttons.

Improved handling of concentrations in scientific notation, and value formatting in general.

In result grids, by right-clicking on column headings, the options can be toggled on or off. Only the selected columns will appear in printed reports, allowing a degree of per-site customisation.

Sample Editor now has Undo button.

Temperature graph can display time axis in real time, not just minutes elapsed since experiment start.

The analysis selection window has been made more compact.

Printed reports now paginate better.

It is now possible to export data from the Rotor-Gene application into any third party application, with the pluggable export filters mechanism. This enables the Rotor-Gene softare to seamlessly integrate with integrated diagnostics systems and facilitate analysis in third-party software such as Matlab and statistical applications.

The Standard Curve formula can now be input directly, to allow import of standard curves averaged over many runs, for example.

In order to further assist with investigation of temperature calibration value corruption, continuous logging has been added. Logging is now also performed while the machine is idle

More detailed logging has been included in order to assist with investigation of temperature calibration value corruption

Some OEMs have been unable to run in virtual mode. This has been rectified.

Some diagrams in the software manual were corrected.

Errors were corrected in 5.0.63 relating to the handling of OEM distribution units.

This software version is for use specifically with instruments with serial numbers 100405 and 100412.

Access Denied errors no longer occur when opening small REX files on Windows NT 4.0

When the operating computer was running for longer than 28 days, an Overflow error could appear on some computers intermittently. This has been fixed in this release.

Enhancements to windows power saving handling in version 5.0.47 caused a software error when running the application under in Windows NT 4.0. This has been corrected.

Fix "Data does not match column type" error when copying and pasting positive and negative controls in the sample editor.

Fix change scale window appearing off-screen when Large Fonts is enabled.

Additional diagnostic information given for troubleshooting logging errors.

Saving profiles in version 4.4 format would fail if touchdown or longrange settings were defined. This has been fixed.

The Reaction Efficiency Threshold option, which excludes samples from analysis determined as non-reactions by the Comparative Quantitation technique, is now disabled by default. This matches behaviour for analysis prior to version 5.0.28. It can be enabled via the Quantitation Settings.

The mechanism used to remember the last quantitation settings selected by the user to be used for subsequent runs was not working. This has been fixed. This means that it will no longer be necessary to manually set ones normalisation settings for individual runs.

A bug would display the incorrect NTC threshold value in the normalisation settings window for Allelic Discrimination and Scatterplot Analysis. This has been fixed.

The highlighted range for auto-find threshold was being displayed incorrectly when the lower bound for the search was set to 0 fluorescence units. This has been fixed.

The standard deviations reported in the software now use the unbiased measure instead of the population standard deviation. This will nominally change error values as reported in the software for CT standard deviations, and within the Comparative Quantitation technique.

Windows Power Saving is now automatically turned off during runs to prevent an unintended entry into standby mode. The computer will still be able to enter standby mode between runs, or when analysing existing runs.

When selecting an NTC threshold of 30%, an error will no longer be displayed requiring the threshold to be set to lower than this value.

The Quantitation Settings box is now more responsive, and handles more intuitively.

Speed of handling experiments with many quantitation and comparative quantitation analyses has been improved.

The auto-stat facility no longer flickers while the mouse is held down when dragging.

Fix comparative quantitation graph not updating during acquisitions.

The mechanism used to save Rotor-Gene settings has been improved. This will help resolve previous issues with Windows NT systems running in a restricted mode.

A bug in version 5.0.20 reintroduced a locale portability issue for REX files. Files saved in this version and version 5.0.28 cannot be transported between different locales. This was due to a bug in a dependent library of the software.

The help file has been updated to include warnings against turning off poor replicates, and information about the 72-well locking ring.

The Auto-Gain feature on the Rotor-Gene 3000 can now go to fractional levels for gain settings. This enables greater background signal reproducibility.

A fault affecting the display of overlaid standard curves has been fixed, when analyses were not using the default standard set.

The rendering of logarithmic scale graphs, such as those used in the Quantitation window has been made more accurate. This makes it easier to visually determine the spacing of replicates without zooming in.

The smoothing algorithm for the melt curve analysis has been enhanced to provide more accuracy for results. This will introduce a shift of up to 0.5 of a degree when analysing existing runs.

The term CV used in Quantitation has been changed to "% Variation" to avoid confusion with the statistical CV. % Variation represents the percentage by which the standard's CT deviates from the expected value.

An error in the labelling of samples in transpose mode in the help description has been fixed.

The Hold Step slider has had the midpoint adjusted.

The experiment name in Experiment Info will now shrink to fit the window.

Auto-Gain optimisation help is now enabled in all parts of the user interface.

If Optical Denature calibration has been defined on a denaturation step, these settings are no longer available under the cycle step to avoid confusion.

It is now always possible to view auto-gain optimisation settings, even after a run.

Users must now define bins before entering genotypes.

Auto-Find Threshold in Quantitation now defaults to looking for a threshold in the lower third of the graph. This reduces the likelihood of finding thresholds in the non- exponential region. This will change previous results for experiments where the found threshold was in the upper two thirds of the graph. To analyse these experiments according to the previous criteria, manually increase the search range.

Fixed Auto-Threshold still running when standards had been defined, but were deselected. An error is now displayed instead.

A new technique to filter NTC's from quantitation results, Reaction Efficiency Threshold has been introduced. This technique uses the reaction efficiency, as measured in Comparative Quantitation to filter NTC's.

Important: This may change results for old experiments, where channels with no detected reaction being eliminated. To preserve the old behaviour, change the Reaction Efficiency Threshold in the Quantitation Settings box to limit at -100%. This cancels out all efficiency threshold calculations.

This technique may not be suitable for low signal-to-noise ratio chemistries, such as LUX primers.

Optical Denature runs are now visualised more accurately, and have a better time estimation.

An improved algorithm is now used to estimate tube temperature during an Optical Denature step.

Optical Denature now uses an improved peak detection algorithm to determine where the reference sample has denatured. This will improve the efficiency of your Optical Denature runs.

The communications code for the Rotor-Gene 3000 has been further enhanced, to handle more types of communications errors.

New Rotor-Gene 3000 models also keep track of the number of runs performed on a machine. This information is now available under the Experiment Info to aid support technicians.

The profile editor has been updated to allow for long hold steps. The number of seconds should be entered manually on the left-hand side of the screen.

Exported data is no longer rounded off to 2 decimal places. This will improve the quality of normalised data when plotted in an external program such as Excel.

Melt Analyses were not being loaded correctly. This has been fixed.

A display error in the genotype window since version 4.6 has been fixed when the first defined bin is not Bin A (for example, when the first bin has been removed).

An error which occurred when loading experiments from a network drive with a UNC path has been fixed.

Melt Genotyping now allows an arbitrary number of bins and genotypes, instead of the previous limit of 5 bins and 6 genotypes.

To improve to quality of melt results, the initial hold for new experiments has been changed to 30 seconds from 15 seconds.

The channel toolbar no longer flickers during Optical Denature steps in the profile. This also corrects a problem where channel names would disappear when performing especially long Optical Denature runs.

Rotor-Gene version 4.4 files are now supported by the Rotor-Gene 5 software.

Minor spelling and grammar corrections have been made to the user manuals and online documentation.

Some user interface bugs related to the toggling between different rotor sizes and Optical Denature have been corrected.

Attempting to copy the "Selected" column would cause an error to be displayed. This has been corrected.

This change is the first instalment in enabling support for temperature verification rotors. This will assist Corbett technical staff in providing verification information for your machine.

Minor corrections were made to typographical errors in Quantitation explanations.

To better organise your protocols, you can add subfolders to the New window. These will appear as icons that you can double-click to view all contained templates.

Once the software has been installed under Administrator mode, regular users can access the software as restricted or power users. This allows the Rotor-Gene softare to integrate with organisations' existing security policies.

Comparative Quantitation was not correctly handling analyses performed on secondary standard sets. This has been fixed.

Loading of Comparative Quantitation analyses has been also fixed.

General speedups to analysis.

Samples can now be toggled on and off in the sample editor.

A new analysis technique, EndPoint Analysis, is now included in the Rotor-Gene software. EndPoint Analysis allows you to perform genotyping and similar analysis on samples which have been already cycled in another thermal cycler.

A new analysis technique, Scatterplot Analysis, is now included in the Rotor-Gene software. Scatterplot analysis allows you to view the expression of two channels on a grid, and map the samples to genotypes.

Experiment files are now signed with a digital signature. This signature ensures that the file has not been tampered with or damaged since it was last saved by the Rotor-Gene software. All files you have saved in previous versions do not have signatures, and so this verification is not available.

An exciting new technique allows you to perform amplification based upon the reaction of a calibrating tube, rather than rely upon timing. The method allows for greater accuracy and cooler denaturations.

Some cycling techniques produce data which decreases exponentially instead of increasing. The Flip Fluorescent Data function allows standard Quantitation analysis to be performed on these experiments.

The Profile Editor for cycling has been reworked to make it even easier to define a profile. A preview graph is now provided for each cycle as well as the entire profile, and can be modified by dragging the graph lines directly instead of entering values.

The Touchdown and Longrange features can now be applied to any point in a cycling step, and are no longer limited to steps 2 and 3, respectively.

Rotor-Gene Help has been converted to Microsoft HTML Help format. This format provides attractive searching and indexing capabilities, and ensures the software manual and online help are up-to-date with the latest developments in the software.

Results in the Rotor-Gene software will now only be provided once the user has completed the relevant manual steps in the analysis. This ensures that the software will never provide incorrect results due to the user omitting these manual steps.

New style settings for graph lines are available, allowing you to specify line thickness and pattern in addition to colour. This is useful for printing graphs for black and white publications.

The Sample Editor window is no longer a fixed size, and can be maximised to use the full size of the screen.

A new Display Option has been added to the View, Display Options section of the software. When selected, a maximum of 6 windows will be displayed. This option is enabled by default in versions 4.6 of the software, however, it can now be deselected for users who have larger screens.

Instead of performing optimisation just before the first acquisition, it is now possible to optimise before the run. The machine will heat to the required temperature, optimise and then start the run.

It is now possible to run an Auto-Optimisation step in a pre-defined template.

The tooltip Auto-Statistics feature has been replaced by a display of the results on the right-hand side of the main window. This eliminates the problem of the tooltip disappearing if one selected the results to analyse too quickly.

The Sybr channel is less effective for performing runs than the FAM/Sybr channel. To encourage users to use the FAM/Sybr channel instead, this option has been removed from the default list. Users may still recreate this channel if required.

Fixed issue loading and saving concentrations less than 1 when reading files on a locale with a comma as a decimal separator.

Fixed auto-reformat feature which ensures that the format chosen by the user can display the given concentrations to enough accuracy. This feature did not function on non-English locales.

Fixed saving of images in MHT report files.

This version can analyse, in real time, data being acquired on versions 4.4.3 and later. This provides enhanced stability when acquiring for Rotor-Gene 2000 users experiencing acquisition card issues.

This release fixes an error encountered when clicking "Skip" while the Rotor-Gene 3000 was cooling during a run, with High-Speed Rotor enabled.

False triggering of safety mechanism on some machines has been corrected.

Support for detection of COM ports has been improved.

Detects chamber lid opening during run and terminates run.

Improved detection of temperature malfunctions to minimise risk of machine damage.

Fix Overflow errors shown on some machines when run for the first time.

If samples have a background level which slopes downwards instead of upward, slope correction in previous versions would display a sharp dip in the sample data around cycle 20. This was because slope correction assumes that the background level of samples will continue to increase or decrease by the same amount as occurs in the first couple of cycles. If the background level decreases each cycle, by cycle 20, some samples may, by this method, have lower than 0 background fluorescence. This caused the normalisation algorithm to set the signal level to 0 as a warning that something was incorrect. The new interpretation sees a downward-sloping background as a level which approaches insignificance over time. By this interpretation, the normalisation has less and less effect as the run increases, which produces more continuous results.

This change will not affect many customers as, most of the time, the background level is creeping up rather than down.

A bug which would cause newer, and not older log archives to be removed first has been fixed. Only the 40 most recent runs should be kept by the software in the archive folder.

When exporting raw data for the FAM/Sybr channels, a filename such as "FAM/Sybr.txt" was being created. This is not a legal filename, and so has been corrected.

Pause markers would not be drawn on a graph when paused under some circumstances. A message would still be displayed in the Experiment Settings window. This has been fixed.

Under some conditions, the software would not resume the run after pausing the Rotor-Gene 3000. This has been fixed.

This version of the software has support built in to enable reading REX files created by the next major software release.

Log Archive files are now stored as ZIP files, to make it easier for distributors to diagnose support issues.

This change improves the handling of Rotor-Gene 2000's still using "Older Style" rotors when performing melts.

It also fixes a bug introduced where the countdown for the first cycle would begin prematurely when skipping the denature step rapidly after commencing the run.

A bug prevented the user from deleting additional standard sets once they had been created. This has been fixed.

When pasting sample names with CONTROL-V in the sample editor, the first cell was being duplicated. This has been fixed.

The Rotor-Gene 2000 will now cycle more accurately when performing 36-well runs with the Older-Style Rotors.

A bug in the temperature handling meant that the temperature graph would not be updated if the Windows was left running for longer than 28 days. The operation of the run would be unaffected.

In addition to the export to excel option, one can now select a range of the result grids in Quantitation and elsewhere, and copy the results.

When performing Auto-Gain optimisation, it was previously possible to set a optimisation fluorescence of 100 fluorescence units. Readings at this level can be off-scale, and so the maximum has been reduced to 99 Fluorescence units to avoid users encountering this problem.

Hold times are now expressed in terms of minutes and seconds instead of just seconds. This improves the readability of longer hold times.

Spelling fix.

The Auto-Gain optimisation drop-down box where one could select to insert additional channels was not being updated when an Auto-Gain setting was removed. This bug did not have any operational impact.

Fix Hold For time of 4 seconds being displayed in temperature window before run is started.

More extensive logging capabilities have been added to the RG-3000 to facilitate troubleshooting. The number of last runs stored has been increased from 20 to 40.

Extra checking added to avoid "Bad Start Byte" problems on Welcome screen and elsewhere.

Improved handling of "Checksum invalid" errors when acquiring data. The software will now retry up to 10 times when encountering this non-fatal error.

Improved handling of non-fatal machine errors. This will enable the run to continue running in cases where the error encountered can be successfully recovered.

The Save As button was broken in the 4.6.66. This was a minor fix applied in version 4.6.67.

When the auto-gain operation cannot find a valid gain, it will select the closest gain. Before version 4.6.70, the software would choose the last gain attempted instead of the closest value.

Customers are now recommended to use locking rings for 72-well runs. The software has been updated to include this recommendation instead of the blank tubes and caps message in the experiment wizard.

Previously Comparative Quantitation calculated its estimated amplification from the raw data. If the data was noisy, this could result in inaccurate amplifications being estimated. The new software applies a 1-cycle window of smoothing to the data before calculating amplification values.

To troubleshoot curve off-scale issues, the software now allows the entry of floating point gain values for the RG-3000. The Auto-Gain functionality still selects integral gain values.

Some RG-3000 machines experienced a timeout problem when set up with a cycling profile which remained on a single temperature for long periods. This has been fixed.

While new models of the RG-3000 included a temperature optimisation mechanism, the software did not yet take into account these corrections to the temperature plot. This change more accurately plots the tube temperature for these machines.

The FAM channel has been discovered to give better results than the Sybr channel. To encourage customers to use the FAM channel instead, the FAM channel has been renamed to FAM/Sybr, and has been made the default acquisition channel.

The FAM channel has been discovered to give better results than the Sybr channel. To encourage customers to use the FAM channel instead, the FAM channel has been renamed to FAM/Sybr, and has been made the default acquisition channel.

To facilitate installation of multiple versions of the software, the Rotor-Gene's default installation folder has been changed to Rotor-Gene 4.6. The desktop icon's text has also been changed to Rotor-Gene 4.6.

The Save As button was broken in the build of 4.6.66. This release fixes this problem.

Sometimes, due to file system locking errors on Windows 2000 systems, it is not possible for the software to manage the Log Archive directory successfully - that is, removing old experiments to make way for new ones, and re-numbering the folders. To fix this limitation, the software has introduced a more robust method of handling the folders, which is less likely to cause file system locking.

This bug has been fixed in this release. The problem was due to the software attempting to load auto optimisation settings for a channel which no longer exists, causing the fault.

This bug can be reproduced by the following steps:
1. Create a new channel.
2. Perform auto-gain optimisation on the channel.
3. Delete the channel.
4. Save the experiment.
5. Go to File, New and open the experiment wizard.
6. Click through to the channel setup page.
7. Click Optimise.
8. The message "Object Variable or With Block Not Set" appears.

An additional algorithm has been added to checking for spikes which corrects for signal dropouts which occur on some machines.

Control is returned to the user immediately when Rotor-Gene shuts down (Requires EPROM upgrade to enable this fix)

Fixes timeout on shutting down machine (Requires EPROM upgrade to enable this fix)

Fixes "Bad Serial Start Byte" errors when starting application after a serial timeout.

Improved recovery from errors during a run. The machine can now be turned on and off during a run and will restore temperature and continue to cycle. The serial link can also be temporarily lost without disrupting the run.

In version 4.6.50 and earlier, the standard curve would always be drawn correctly where standards were defined. When standards were defined and then disabled, however, the last standard curve would still remain displayed. The curve is now correctly hidden, and the standard curve formula written as "N/A". No calculation of concentrations would occur, so the main impact of this defect was a display anomaly. An anomaly where it was possible to create a standard curve using 1 standard instead of the minimum 2 has also been rectified. Where only 1 standard is defined, the standard curve formula is now shown as "N/A".

Customer feedback indicated that the adjustment of standard curves was a process that occurred with too little transparency. Rules such as the adjustment of imported curves to pass through references in the target experiment were not well understood. This release provides information about exactly what operation will be performed during an import, and enforces "best practice" with relation to standard curves. In particular, importing a standard curve without a reference standard now produces a warning.

On some computers, the new log handler could occasionally generate a permission denied error before a run. This error has been suppressed and a message placed in the experiment's messages content. This fix will also improve reliability on networked systems where the drives can temporarily become unavailable.

The statistics window has been modified to include R and R-squared values. The R-squared value indicates the percentage of the results explained by the hypothesis. For example, an R-squared value of 0.94 means that our assumption that the standards match their given concentrations is suggested by 94% of our data, and that only 6% does not agree with this hypothesis.

The statistics are now included in reports and will be exported to JPEG files.

When performing a run, if the analysis window was open before data was acquired, then data was acquired for the first time, a channel would appear in the raw channels selector in the top of the screen but it would not appear in the analysis window. It was necessary to close the analysis window and then re-open it. This has been fixed.

The new "Help, Send Support Email" menu option allows emailing of all log files, and experiment in one file to aid Corbett Research support technicians in resolving customer issues.

Melt Curve Analysis now has a Full Report option to match that available in Quantitation analysis. The Full Report contains all graphs related to the analysis as well as the profile information.

The screen to modify the NTC Threshold has been made more responsive, making it easier to view the effects a certain threshold level will have on results.

A bug was introduced in 4.6.44 which would not save the NTC Percentage Threshold correctly. This is fixed in this release.