Rotor-Gene Version History

The software now takes into account the temperature of the ambient air surrounding your instrument. What this means is you can have confidence that the temperature is correct in the environment you are running it in.

MT-PCR is a 2-step process for gene profiling or mutation detection from very small amounts of RNA. It can be configured to produce a gene expression profile from RNA extracted from a single section of formaldehyde fixed paraffin embedded specimens or from single cell amounts of RNA. The MT-PCR Analysis allows users who have purchased MT-PCR kits to intepret their results based on the contents of their kit. Visit http://www.corbettdiagnostics.com for more details.

HRM now uses the 510nm filter, previously it was using the 510hp filter. This has been changed due to significant improvements in accuracy.

When exporting a quantitation analysis to Excel, the given concentration now matches to value displayed in the software. The values were completely off in previous versions.

Melt steps of 0.02 degrees have now been allowed in the software.

The reported temperature reading in the temperature display now matches what is seen in the temperature graph. Previously these values did not coincide.

Melt steps have always had an initial hold time to improve the stability of the temperature before melting, this improved the accuracy of the final melt. This initial hold has now been replaced with a new pre-melt conditioning step that improves on this by extending the melt to start slightly earlier; the end result being the melt temperature is even more accurate than before.

In some situations performing an auto gain on the first acquisition would result in spiked data on the very first data point. This problem has now been rectified.

The software previously only supported COM ports up to COM9. We have improved this mechanism such that it can support COM ports up to COM256.

Previously it was not obvious when the default normalisation settings were being saved. Improvements have now been made so that the user is in complete control over when the normalisation settings are saved.

The quick start wizard has had improvements to make it faster for the user to get their runs started.

Channel names have previously used dye specific naming FAM, JOE, ROX, Cy5 however we have changed this to a more generic naming system Green, Yellow, Orange, Red.

If the existing COM port is not responding, the software now does an auto detect to find the correct one on start up.

The speed the the machine runs when in virtual mode has been increased to make things easier when demonstrating the software.

The multi-channel melt report now shows the correct genotyping results when not all samples are selected.

The minimum hold time on the initial melt hold has been decreased to 1 second. This will allow users to speed up their run times, however should be used with caution as having a initial hold that is too short can result in spikes at the beginning of melt data.

If Optical Denature calibration is set on a hold step, the hold steps original settings are no longer overridden. This means changing a Optical Denature calibration back into a hold step will restore the original hold parameters.

The software was only able to support a very limited number of genotypes, excessive amounts causing it to crash. This has now been rectified.

Samples that cross the quantitation threshold more than once have an corresponding error displayed in the result table.

On Italian operating systems statistics displayed in the reports are now formatted correctly.

In the quantitation analysis, samples that cross the threshold more than once are now treated as invalid and no CT value is displayed. This behaviour is now strictly enforced.

Users of this software release will be able to run specially created templates to adjust the configuration of their instrument, under the instruction of the manufacturer's technical staff. This will assist in providing more streamlined customer support.

Functionality has been added to allow for the import and export of specific analysis settings, available when viewing the main window of an analysis. The specific analysis settings, such as bin and genotype definitions, are saved to a file and may then be loaded in a different run.

A new template has been added which is available for use through the wizard. This template is not based on any specific chemistry, allowing the use of chemistry that is not currently catered for in the wizard. All analysis types are available within.

Added button to save setup as new template.

Added gain optimisation button.

Added ability to open a template in another folder.

Possibility of export to multiple graphics formats, including GIF, JPEG, PNG and WMF.

New "Copy to Clipboard" command to allow pasting of graphs directly into PowerPoint presentations.

Allow export to TeeChart Office.

A hybridisation step has been introduced for selection in the profile. The hybridisation step replaces the previous reverse melt step.

It is now possible to export Quantitative data directly to LinReg. Since LinReg uses normalised quantitation data, you will need to create a quantitation analysis before exporting.

Added option to analysis selection window to show hidden analysis types.

Added extra comparative quantitation statistics.

Fix Melt report filter settings being incorrectly reported.

Fixed resizing of sample selector control.

Option to expand/contract added to quantitation docking bar.

Reporting window widened.

Fixed page breaks for letter-sized reports.

Enhanced accuracy of temperature graph during acquisition.

For multiplex reactions where multiple pages use identical sample names, an option is available via the sample editor to automatically keep sample names and colours up-to-date.

Integration into TeeChart Office has been provided so that graphs may now be selected for edit in TeeChart Office directly from the Rotor-Gene software. TeeChart Office is a free download from our website. This package provides capabilities to change line styles, add legends to graphs and change fonts as required by certain publications.

It is now possible to toggle the sample ID format in the sample editor.

It is now possible to set the touchdown accuracy between 0.1 and 2 degrees per step.

The ability to specify names and ranges for melt bins has been introduced. Also, bin details may now be edited.

The ability has been added to specify limits on the gains to be scanned during the auto-gain optimisation process.

The SYBR Green template now automatically performs an auto-gain optimisation on the first tube position. This optimisation can be configured through the Quick Start wizard.