Welcome to NucleoLink Application Guide from Nunc.

The information contained herein is intended for research purposes only to guide the investigator in the utilization of the NucleoLink surface.

Technical support is one of the strong commitments from Nunc to our customers. This CD-rom is meant as an advanced supplement to the printed TechNotes, Bulletins and scientific articles we publish. The Application Guide is designed to add an extra dimension to your knowledge about NucleoLink, both as a new user, and if you use NucleoLink on a routine basis in your laboratory.

Through our daily work in the laboratories at Nunc A/S in Denmark we gain detailed information on our innovative products like NucleoLink. It is very important that our customers worldwide gain from our experience. The purpose of this application guide is therefore to transmit the information in a format which makes it an easy tool for everybody.

If you are unfamiliar with NucleoLink Strips please turn to NucleoLink Introduction ( ) where the basic concept of NucleoLink is explained.

On the CD-ROM you will find all the available knowledge concerning NucleoLink Strips, and DIAPOPS ( ). This includes:

• Physical facts about NucleoLink Strips ( )
• Product information ( )
• Trouble Shooting guide ( )
• DIAPOPS and PCR* introduction, information, and optimization.
• General information concerning the use of NucleoLink Strips
• DIAPOPS procedure ( ).
• Data from the optimization experiments for optimal conditions in the DIAPOPS procedure ( ).
• Publications

All data on the CD-ROM are available through the 'Knowledge Base Table of Contents'. However, much of the information can also be found through the DIAPOPS Trouble Shooting, or through the DIAPOPS procedure.

The Application Guide is designed to be used with an Internet browser. The Microsoft® Explorer is provided on this CD-ROM and is ready to be installed. However, if you already have a browser installed on your computer, you can use that.

Your evaluation of the NucleoLink Application Guide and your suggestions for changes and additions to the Guide are very important for us. Therefore, please spend a minute or two to fill in the Questionnaire ( ) and fax it to us. We would appreciate receiving this questionnaire when you have made yourself familiar with the Application Guide. If you have been looking for something which could not be found in the Application Guide or if you have suggestions for improvements, we would also like to hear from you.

We hope you will enjoy this new tool. Go ahead and browse!

Mention of specific products in the NucleoLink Application Guide does not constitute an endorsement or a specific recommendation for use of the product. All technical questions must be directed to the company in question.

*The Polymerase Chain Reaction (PCR) process is covered by US patents owned by Hoffmann-La Roche, Inc and F. Hoffmann-La Roche Ltd.