indicates enzymes that are not currently commercially available
Single letter code:
R = G or A
Y = C or T
W = A or T
M = A or C
K = G or T
S = C or G
H = A, C or T
V = A, C or G
B = C, G or T
D = A, G or T
N = G, A, T or C
Specificity 5'=>3' Enzyme AA^CGTT Psp1406I A^AGCTT HindIII AAT^ATT SspI ^AATT TasI A^CATGT BspLU11I A^CCGGT BshTI ACCNNNNNNGGT HgiEII ACCTGC(N)4/8^ BveI A^CCWGGT SexAI A^CGCGT MluI ACGGC(N)12/14^ BceAI ACGGG BscGI A^CGT MaeII ACGT^ TaiI ^10/15(N)AC(N)4GTAYC(N)12/7^ BaeI ACN^GT TaaI ACNNNNNCTCC BsaXI A^CRYGT AflIII A^CTAGT Bcul ACTGGG(N)5/4^ BfiI ACTGG(N)1/-1^ BseNI ^9/15(N)AC(N)6TGG(N)14/8^ CjeI A^GATCT Bglll AGC^GCT Eco47III AG^CT Alul AGG^CCT Eco147I AG^GWCCT Sse8647I AGT^ACT ScaI AT^CGAT Bsu15I ATG^CAT BfrBI ATGCA^T Mph1103I AT^TAAT VspI ATTT^AAAT SmiI
Specificity 5'=>3' Enzyme CAACAC BsbI CAARCA(N)11/9^ Tth111II C^AATTG MunI CACCCA(N)11/9^ TaqII CACCTGC(N)4/8^ AarI CACGAG-5/-1^ BssI CAC^GTC BtrI CAC^GTG Eco72I CACNN^NNGTG OliI CACNNN^GTG AdeI ^0/2(N)CACTGC BtsI CAGCTC(N)7/11 AceIII CAG^CTG Pvull CAGNNN^CTG CaiI CASTG(N)2/-7^ TspRI CAYNN^NNRTG MslI CA^TATG NdeI ^0/2(N)CATCC BseGI ^13/9(N)CATCC FokI 14/10(N)CATCC StsI CATG^ NlaIII C^ATG CviAII ^0/2(N)CATTGC BseMI ^-1/1(N)CCAGT BseNl ^8/14(N)CCA(N)6GT(N)15/9^ CjeI ^7/12(N)CCA(N)6GTC(N)13/8^ Bsp24I ^7/13(N)CCA(N)7TC(N)14/8^ CjePI CCANNNN^NTGG Van91I CCANNNNN^NNNNTGG XcmI CCANNNNN^NTGG BstXI CCATC BccI C^CATGG NcoI C^CRYGG DsaI CCCACA(N)12/9^ RleAI ^4/5(N)CCCAGT BfiI CCCGC(N)4/6^ SmuI C^CCGGG Cfr9I CCC^GGG Smal CCCG(N)4/8^ Sth132I CCCGT BscGI CCGC-3/-1^ AciI CCGC^GG Cfr42I CCG^CTC MbiI C^CGG HpaII, MspI ^CCNGG BssKI CC^NGG Bme1390I C^CNNGG BseDI CCNNNNN^NNGG BseLI C^CRYGG DsaI ^CCSGG EcoHI CC^SGG BcnI C^CTAGG XmaJI CCTCAGC-5/-2^ BbvCI CCTC(N)7/6^ MnlI CCTGCA^GG SdaI CC^TNAGC Bpu10I CC^TNAGG Eco81I CCTNN^NNNAGG XagI ^CCWGG EcoRII CC^WGG MvaI C^CWWGG Eco130I CGAACG UbaPI CGAT^CG PvuI ^10/12(N)CGA(N)6TGC(N)12/10^ BcgI CG^CCGGCG Sse232I ^CGCG SelI CG^CG Bsh1236I C^GGCCG Eco52I ^-1/-5CGGCCR GdiII ^8/4(N)CGGG Sth132I CG^GWCCG Cpol CGRY^CG Bsh1285I C^GTACG Pfl23II CGTCTC(N)1/5^ Esp3I CGTTCG UbaPI CGWCG^ Hpy99I CMG^CKG NspBII CR^CCGGYG SgrAI CTACGA Pfl1108I C^TAG MaeI CTCAG(N)10/8^ BseMII ^14/16(N)CTCCAG GsuI ^8/10(N)CTCCTC BseRI C^TCGAG XhoI CTC^GAG SciI ^14/16(N)CTCAAG Bce83I CTCGTG-5/-1^ BssSI CTCTTC(N)1/4^ Eam1104I CTGAAG(N)16/14^ Eco57I ^14/16(N)CTGCAC BsgI CTGCA^G Pstl CTGGAC BspGI ^8/10(N)CTGAG BseMII CTGGAG(N)16/14^ GsuI CTGRAG(N)16/14^ Eco57MI C^TNAG DdeI C^TRYAG BfmI C^TTAAG BspTI ^14/16(N)CTTCAG Eco57I CTTGAG(N)16/14^ Bce83I C^TYRAG SmlI ^14/16(N)CTYCAG Eco57MI C^YCGRG Eco88I CYCGR^G Nli3877I
Specificity 5'=>3' Enzyme GAACCA DrdII ^7/12(N)GAAC(N)5CTC(N)13/8^ PpiI ^7/12-13(N)GAAC(N)6TCC(N)12-13/7^ AloI GAAGAC(N)2/6^ BpiI GAAGAC(N)7/11^ Bbr7I ^4/1(N)GAAGAG Eam1104I ^4/1(N)GAAGAGC SapI GAAGA(N)8/7^ MboII GAANN^NNTTC PdmI GAATGC(N)1/-1^ Mva1269I G^AATTC EcoRI ^8/13-14(N)GAY(N)5VTC(N)13-14/8^ Hin4I ^0/-3GACCC SimI GACCGA(N)11/9^ TaqII GACGC(N)5/10^ HgaI GACGT^C AatII GAC^GTG BtrI ^5/5(N)GACTC SchI ^5/4(N)GACTC PleI GACN^NNGTC PsyI GACNN^NNGTC BoxI GACNNN^NNGTC Eam1105I GACNNNN^NNGTC AasI ^8/13(N)GAC(N)6TGG(N)12/7^ Bsp24I ^5/1(N)GAGAC Alw26I ^5/1(N)GAGACC Eco31I ^5/1(N)GAGACG Esp3I GAG^CGG MbiI GAG^CTC Ecl136ll GAGCT^C SacI ^11/7(N)GAGCTG AceIII ^6/7(N)GAGG MnlI GAGTC(N)5/5^ SchI GAGTC(N)4/5^ PleI ^8/13(N)GAG(N)5CTC(N)13/8^ BplI GAGGAG(N)10/8^ BseRI ^8/13(N)GAG(N)5GTTC(N)12/7^ PpiI G^ANTC HinfI GAYNNNNNRTC HaeIV GAT^ATC Eco32I ^GATC Bsp143I, MboI Gm6A^TC DpnI GAT^C BspKT6I GATC^ ChaI ^5/4(N)GATCC BspPI ^9/5(N)GATGC LweI ^6/4(N)GATGC BscAI GATGG BccI ^8/14(N)GA(N)7TGG(N)13/7^ CjePI GATNN^NNATC BseJI G^AWTC TfiI GCAATG(N)2/0^ BseMI GCAGC(N)8/12^ BseXI GCAGGTG AarI ^8/4(N)GCAGGT BveI GCAGTG(N)2/0^ BtsI GCANNNN^NTGC BstAPI GCANNNNN^TGC ApaBI GCATC(N)5/9^ LweI GCATC(N)4/6^ BscAI ^10/12(N)GCA(N)6TCG(N)12/10^ BcgI GCATG^C PaeI ^-1/1(N)GCATTC Mva1269I GCCC^GGGC SrfI G^CCGGC NgoMIV GCC^GGC PdiI ^14/12(N)GCCGT BceAi GCCNNNN^NGGC Bgll GCGAT^CGC SgfI G^CGC Hin6I GCG^C HhaI G^CGCGC PauI GCGG-3/-1^ AciI GC^GGCCGC NotI ^6/4(N)GCGGG SmuI GC^NGC SatI GCN^NGC Cac8I GCNNNNN^NNGC HpyF10VI ^10/5(N)GCGTC HgaI GCSG^C TauI G^CTAGC NheI GCTAG^C AceII GCTCTTC(N)1/4^ SapI GCTGAGG-5/-2^ BbvCI ^12/8(N)GCTGC BseXI GC^TNAGC Bpu1102I GC^TNAGG Bpu10I G^CWGC TseI GDGCH^C SduI ^7/10(N)GGAGNNNNNGT(N)12/9^ BsaXI G^GATCC BamHI GGATC(N)4/5^ BspPI ^7/12-13(N)GGA(N)6GTTC(N)12-13/7^ AloI ^5/6(N)GGATAC BfuI GGATG(N)2/0^ BseGI GGATG(N)9/13^ FokI GGATG(N)10/14^ StsI GG^CC BsuRI GGCCGG^CC FseI GGCCNNNN^NGGCC SfiI G^GCGCC KasI GG^CGCC NarI GGC^GCC EheI GGCGC^C BbeI GG^CGCGCC AscI GGCGGA(N)11/9^ EciI GGGAC(N)10/14^ BsmFI G^GGCCC Bsp120I GGGCC^C ApaI GGGCMC BmgI GGGTC-3/0^ SimI GG^GWCCC SanDI ^GGNCC UnbI G^GNCC Cfr13I GGNC^C FmuI GGN^NCC BspLI G^GYRCC BshNI G^GTACC Acc65I GGTAC^C KpnI GGTCTC(N)1/5^ Eco31I GGTGA(N)8/7^ HphI GGTGA(N)8/8^ SspD5I G^GTNACC Eco91I ^GGWCC VpaK11AI G^GWCC Eco47I GKGCCC BmgI GKGCM^C BseSI GR^CGYC Hin1I GRGCY^C Eco24I ^7/12(N)GRTAC(N)4GT(N)15/10^ BaeI G^TAC Csp6I GT^AC RsaI GTA^TAC Bst1107I GTATCC(N)6/5^ BfuI GTCCAG BspGI ^14/10(N)GTCCC BsmFI G^TCGAC SalI GTCTC(N)1/5^ Alw26I ^6/2(N)GTCTTC BpiI ^11/7(N)GTCTTC Bbr7I G^TGCAC Alw44I GTGCAG(N)16/14^ BsgI GTGTTG BsbI GT^MKAC XmiI ^GTNAC MaeIII GTN^NAC Hpy8I ^GTSAC NmuCI ^18/20(N)GTYGGA MmeI GTY^RAC HincII GTT^AAC KspAI GTTT^AAAC MssI GWGCW^C Alw21I
Specificity 5'=>3' Enzyme R^AATTY XapI RCATG^Y XceI R^CCGGY Cfr10I R^GATCY PsuI RGC^GCY LpnI RGCGC^Y Bsp143II RG^CY CviJI RG^GWCCY Psp5II RG^GNCCY EcoO109I RGGNC^CY PssI RTGC^GCAY FspAI Y YAC^GTR BsaAI YGGCCG-5/-1^ GdiII Y^GGCCR CfrI
Specificity 5'=>3' Enzyme TAC^GTA Eco105I ^7/8(N)TCACC HphI ^8/8(N)TCACC SspD5I T^CATGA PagI ^9/11(N)TCCGCC EciI T^CCGGA Kpn2I T^CCNGGA PfoI TCCRAC(N)20/18^ MmeI T^CGA TaqI TCG^CGA Bsp68I TCGTAG Pfl1108I TCN^GA Hpy188I TC^NNGA Hpy188III T^CTAGA Xbal ^7/8(N)TCTTC Mboll T^GATCA BclI TG^CA CviRI TGC^GCA NsbI TGG^CCA MlsI ^9/11(N)TTCGGTC TaqII ^9/11(N)TTGGGTG TaqII TGGTTC DrdII ^9/11(N)TGYTTG Tth111II T^GTACA Bsp1407I ^9/12(N)TGTGGG RleAI T^TAA Tru1I TTAAT^TAA PacI TTA^TAA PsiI TT^CGAA Bsp119I TTT^AAA DraI W W^CCGGW BsaWI WGG^CCW HaeI W^GTACW TatI
 Home |
Search |
Contacts |
Order |
Catalog |
Support |
Updated October 22, 2002 12:44