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S

U

P

P

O

R

T

 

Commercial Restriction Endonucleases Sorted by Generated DNA Ends

 Note 

Asymmetric sequences are indicated by *.

Single letter code:
R = G or A; 
Y = C or T; 
W = A or T; 
M = A or C; 
K = G or T; 
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.

Enzymes Generating Blunt Ends

Recognition
sequence
Enzyme
AAT^ATT SspI
AGC^GCT Eco47III
AG^CT AluI
AGG^CCT Eco147I
AGT^ACT ScaI
ATTT^AAAT  SmiI
CACGTC(-3/-3)^* BtrI
CAC^GTG Eco72I
CACNN^NNGTG OliI
CAG^CTG PvuII
CAYNN^NNRTG MslI
CCC^GGG SmaI
CCGCTC(-3/-3)^* MbiI
CG^CG Bsh1236I
CMG^CKG NspBII
GAANN^NNTTC PdmI
GACGTG(-3/-3)^* BtrI
GACNN^NNGTC BoxI
^(5/5)GACTC* SchI
GAGCGG(-3/-3)^* MbiI
GAG^CTC Ecl136II
GAGTC(5/5)^* SchI
GAT^ATC Eco32I
Gm6A^TC DpnI
GATNN^NNATC BseJI
GCCC^GGGC SrfI
GCC^GGC PdiI
GCN^NGC Cac8I
GG^CC BsuRI
GGC^GCC EheI
GGN^NCC BspLI
GT^AC RsaI
GTA^TAC Bst1107I
GTN^NAC Hpy8I
GTY^RAC HincII
GTT^AAC KspAI
GTTT^AAAC MssI
YAC^GTR BsaAI
RG^CY CviJI
RTGC^GCAY FspAI
TAC^GTA Eco105I
TCG^CGA Bsp68I
TG^CA CviRI
TGC^GCA NsbI
TGG^CCA MlsI
TTA^TAA PsiI
TTT^AAA DraI

 

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Updated September 18, 2002 14:40