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P

P

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R

T
 

Alphabetic List of Fermentas Restriction Endonucleases

 Note 

List of commercial restriction endonucleases and their isoschizomers see.

Single letter code:
R = G or A; 
Y = C or T; 
W = A or T; 
M = A or C; 
K = G or T; 
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.

AB    CDEF    GHKLMNO    PRS   TVX   Nicking enzyme

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #
new AarI AarI CACCTGC(N)4/8^ ER1581/2
new AasI DrdI GACNNNN^NNGTC ER1721/2
  AatII  AatII  GACGT^C ER0991/2
  Acc65I  KpnI (GGTAC^C) G^GTACC ER0901/2
  AdeI  DraIII  CACNNN^GTG ER1231/2
  AloI  AloI  ^7/12-13(N)GAAC(N)6TCC(N)12-13/7^ ER1491/2
  AluI  AluI  AG^CT ER0011/2
  Alw21I  HgiAI  GWGCW^C ER0021/2
  Alw26I  BsmAI  GTCTC(N)1/5^ ER0031/2
  Alw44I  ApaLI  G^TGCAC ER0041/2
  ApaI  ApaI  GGGCC^C ER1411/2
  BamHI  BamHI  G^GATCC ER0051/2/3
  BclI  BclI  T^GATCA ER0721/2
  BcnI  CauII  CC^SGG ER0061/2
  BcuI  SpeI  A^CTAGT ER1251/2
new BfiI BfiI ACTGGG(N)5/4^ ER1591/2
  BfmI SfeI  C^TRYAG ER1161/2
  BfuI  BciVI  GTATCC(N)6/5^ ER1501/2
  BglI  BglI  GCCNNNN^NGGC  ER0071/2
  BglII  BglII  A^GATCT ER0081/2
  Bme1390I  ScrFI  CC^NGG ER1421/2
  BoxI  PshAI  GACNN^NNGTC ER1431/2
  BpiI  BbvII  GAAGAC(N)2/6^ ER1011/2
  BplI  BplI  ^8/13(N)GAG(N)5CTC(N)13/8^ ER1311/2
  Bpu10I  Bpu10I  CCTNAGC-5/-2^ ER1181/2
  Bpu1102I  EspI  GC^TNAGC ER0091/2
  BseDI  SecI  C^CNNGG ER1081
  BseGI  FokI (GGATG(N)9/13^) GGATG(N)2/0^ ER0871/2
new BseJI BsaBI GATNN^NNATC ER1711
  BseLI  BsiYI  CCNNNNN^NNGG ER1201/2
  BseMI  BsrDI  GCAATG(N)2/0^ ER1261/2
  BseMII  BseMII  CTCAG(N)10/8^ ER1401/2
  BseNI  BsrI  ACTGG(N)1/-1^ ER0881/2
  BseSI  BseSI  GKGCM^C ER1441/2
  BseXI  BbvI  GCAGC(N)8/12^ ER1451/2
  Bsh1236I  FnuDII  CG^CG ER0921/2
  Bsh1285I  McrI  CGRY^CG ER0891
  BshNI  HgiCI  G^GYRCC ER1001/2
  BshTI  AgeI  A^CCGGT ER1461/2
  Bsp68I  NruI  TCG^CGA ER0111/2
  Bsp119I  AsuII  TT^CGAA ER0121
  Bsp120I  ApaI (GGGCC^C)  G^GGCCC ER0131/2/3
  Bsp143I  MboI  ^GATC ER0781/2
  Bsp143II  HaeII  RGCGC^Y  ER0791/2
  Bsp1407I  Bsp1407I  T^GTACA ER0931/2
  BspLI  NlaIV  GGN^NCC ER1151/2
  BspPI  BinI  GGATC(N)4/5^ ER1321/2
  BspTI  AflII  C^TTAAG ER0831/2
  Bst1107I  SnaI  GTA^TAC ER0701/2
  BstXI  BstXI  CCANNNNN^NTGG ER1021/2 
  Bsu15I  ClaI  AT^CGAT ER0141/2
  BsuRI  HaeIII  GG^CC ER0151/2/3
 new BveI  BspMI  ACCTGC(N)4/8^ ER1741

CDEF

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #
  CaiI  AlwNI  CAGNNN^CTG ER1391/2
  CfrI  CfrI  Y^GGCCR ER0161/2
  Cfr9I  SmaI (CCC^GGG) C^CCGGG ER0171/2
  Cfr10I  Cfr10I  R^CCGGY ER0181/2
  Cfr13I  AsuI  G^GNCC ER0191/2
  Cfr42I  SacII  CCGC^GG ER0201/2
  CpoI  RsrII  CG^GWCCG ER0741/2
  Csp6I  RsaI (GT^AC) G^TAC ER0211
 new DpnI  DpnI  Gm6A^TC ER1701/2
  DraI  AhaIII  TTT^AAA ER0221/2
  Eam1104I  Ksp632I  CTCTTC(N)1/4^ ER0231/2
  Eam1105I  Eam1105I  GACNNN^NNGTC ER0241/2
  Ecl136II  SacI (GAGCT^C)  GAG^CTC ER0251/2
  Eco24I  HgiJII  GRGCY^C ER0281
  Eco31I  Eco31I  GGTCTC(N)1/5^ ER0291/2
  Eco32I  EcoRV  GAT^ATC ER0301/2/3
  Eco47I  AvaII  G^GWCC ER0311/2
  Eco47III  Eco47III  AGC^GCT ER0321/2
  Eco52I  XmaIII  C^GGCCG ER0331/2 
  Eco57I  Eco57I  CTGAAG(N)16/14^ ER0341/2
new Eco57MI  Eco57MI  CTGRAG(N)16/14^ ER1671/2
  Eco72I  PmaCI  CAC^GTG ER0361/2
  Eco81I  SauI  CC^TNAGG ER0371/2
  Eco88I  AvaI  C^YCGRG ER0381
  Eco91I  BstEII  G^GTNACC ER0391/2
  Eco105I  SnaBI  TAC^GTA ER0401/2
  Eco130I  StyI  C^CWWGG ER0411
  Eco147I  StuI  AGG^CCT ER0421/2
  EcoO109I  DraII  RG^GNCCY ER0261
  EcoRI  EcoRI  G^AATTC  ER0271/2/3
  EheI  NarI (GG^CGCC) GGC^GCC ER0441
  Esp3I  Esp3I  CGTCTC(N)1/5^ ER0451/2
 new FspAI  FspAI  RTGC^GCAY ER1661/2

GHKLMN

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #
  GsuI  GsuI  CTGGAG(N)16/14^ ER0461/2
  Hin1I  AcyI  GR^CGYC ER0471/2
new Hin4I Hin4I ^8/13-14(N)GAY(N)5VTC(N)13-14/8^ ER1601/2
  Hin6I  HhaI (GCG^C) G^CGC ER0481/2
  HincII  HindII  GTY^RAC ER0491/2
  HindIII  HindIII  A^AGCTT ER0501/2/3
  HinfI  HinfI  G^ANTC ER0801/2/3
  HpaII  HpaII  C^CGG ER0511/2
  HphI  HphI  GGTGA(N)8/7^ ER1101/2

new 

 Hpy8I  MjaIV  GTN^NAC ER1571/2
new HpyF10VI MwoI GCNNNNN^NNGC ER1731/2
  KpnI  KpnI  GGTAC^C ER0521/2/3
  Kpn2I  BspMII  T^CCGGA ER0531/2
  KspAI  HpaI  GTT^AAC ER1031/2
new LweI SfaNI GCATC(N)5/9^ ER1621/2
  MbiI  BsrBI  GAGCGG-3/-3^ ER1271
  MboI  MboI  ^GATC ER0811/2
  MboII  MboII  GAAGA(N)8/7^  ER0821/2
  MlsI  BalI  TGG^CCA ER1211/2
  MluI  MluI  A^CGCGT ER0561/2
  MnlI  MnlI  CCTC(N)7/6^ ER1071/2
  Mph1103I  AvaIII  ATGCA^T ER0731/2
  MspI  HpaII  C^CGG ER0541/2
  MssI  PmeI  GTTT^AAAC ER1341/2
  MunI  MfeI  C^AATTG ER0751/2
  MvaI  EcoRII (^CCWGG) CC^WGG ER0551/2
  Mva1269I  BsmI  GAATGC(N)1/-1^ ER0961/2
  NcoI  NcoI  C^CATGG ER0571/2
  NdeI  NdeI  CA^TATG ER0581/2
  NheI  NheI  G^CTAGC ER0971/2
  NmuCI  Tsp45I  ^GTSAC ER1511/2
  NotI  NotI  GC^GGCCGC ER0591/2/3
  NsbI  MstI  TGC^GCA ER1221/2
new OliI OliI CACNN^NNGTG ER1631/2

PRS

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #
  PaeI  SphI  GCATG^C ER0601/2
  PagI  BspHI  T^CATGA ER1281/2
  PauI  BsePI  G^CGCGC ER1091/2
  PdiI  NaeI  GCC^GGC ER1521/2
  PdmI  XmnI  GAANN^NNTTC ER1531/2
  Pfl23II  SplI  C^GTACG ER0851
 new PfoI  PfoI  T^CCNGGA ER1751
  PpiI  PpiI  ^7/12(N)GAAC(N)5CTC(N)13/8^ ER1541/2
  Psp5II  PpuMI  RG^GWCCY ER0761/2
  Psp1406I  AclI  AA^CGTT ER0941/2
  PstI  PstI  CTGCA^G ER0611/2/3
  PsuI  XhoII  R^GATCY ER1551/2
  PsyI  Tth111I  GACN^NNGTC ER1331/2
  PvuI  PvuI  CGAT^CG ER0621/2
  PvuII  PvuII  CAG^CTG ER0631/2/3
  RsaI  RsaI  GT^AC ER1121/2
  SacI  SacI  GAGCT^C ER1131/2/3
  SalI  SalI  G^TCGAC ER0641/2/3
new SatI Fnu4HI GC^NGC ER1641/2
  ScaI  ScaI  AGT^ACT ER0431/2
  SchI  PleI (GAGTC(N)4/5^) GAGTC(N)5/5^ ER1371
  SdaI  Sse8387I CCTGCA^GG ER1191/2
  SduI  SduI  GDGCH^C ER0651
  SmaI  SmaI  CCC^GGG ER0661/2/3
  SmiI  SwaI  ATTT^AAAT ER1241/2
 new SmuI  FauI  CCCGC(N)4/6^ ER1691/2
  SspI  SspI  AAT^ATT  ER0771/2

TVX

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #

 

 TaaI  Tsp4CI  ACN^GT ER1361/2
  TaiI  MaeII (A^CGT) ACGT^ ER1141/2
  TaqI  TaqI  T^CGA ER0671/2
  TasI  TspEI  ^AATT ER1351/2
  TatI  TatI  W^GTACW ER1291/2
new TauI TauI GCSG^C ER1651/2
  Tru1I  MseI  T^TAA ER0981/2/3
  Van91I  PflMI  CCANNNN^NTGG ER0711/2
  VspI  VspI  AT^TAAT ER0911/2
  XagI  EcoNI  CCTNN^NNNAGG ER1301/2
  XapI  ApoI  R^AATTY ER1381/2
  XbaI  XbaI  T^CTAGA ER0681/2/3
  XceI  NspI  RCATG^Y ER1471/2
  XhoI  XhoI  C^TCGAG ER0691/2/3
  XmaJI  AvrII  C^CTAGG ER1561/2
  XmiI  AccI  GT^MKAC ER1481/2

  Fermentas
Enzyme		 Prototype Specificity
5'=>3'		 Catalog #

new

 N.Bpu10I  N.Bpu10I  GC^TNAGG
CG  ANTCC		 ER1681/2

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Updated October 22, 2002 11:24