Contents
DIAPOPS General Information	Solid phase primer
DIAPOPS Miscellaneous Information	NucleoLink
PCR*	General Information
Background Signals	Publications
Detection Systems
DIAPOPS General Information
DIAPOPS introduction DIAPOPS Animation Applications or systems tested with DIAPOPS Advice for optimization of DIAPOPS A comparison of PCR products in liquid - and solid phase
DIAPOPS Miscellaneous Information
Preparation of buffers for the DIAPOPS procedure Rehybridization to the solid phase PCR product Pre-PCR blocking of coated NucleoLink Strips DIAPOPS signal as a function of denaturation method of solid phase amplicon after PCR Probe concentration and probe labeling in DIAPOPS The washing steps in the DIAPOPS procedure DIAPOPS Procedure
PCR*
General PCR introduction Animation Hints for optimization of PCR Selection of primers for PCR and DIAPOPS Carry-over problems Hot-Start
Background Signals
Background problems not coming from solid phase primer Background originating from the solid phase primer A case-story of a background problem
Detection Systems
Alkaline phosphatase Horseradish peroxidase Summary of enzymes and substrates tested in DIAPOPS
Solid phase primer
EDC coupling reaction Measurement of the number of solid phase primers, and the relationship to the solid phase amplification Results from optimization of the procedure for covalent binding of the solid phase primer to the surface of NucleoLink DIAPOPS results as a function of a linker in the 5'-end of the solid phase primer EDC from different sources and effect of storage of EDC Heat stability of the solid phase primer Stability of the mix of primer and EDC in different buffers Phosphorylation of the solid phase primers
NucleoLink
Physical stability and form of the NucleoLink Strips General handling of NucleoLink Strips in DIAPOPS Storage ability of NucleoLink Strips
General Information
Thermal cyclers General handling of oligonucleotides Questionnaire A list of abbreviations and terms Scientific reference list Index to NucleoLink Application Guide Introduction to NucleoLink Application Guide Introduction to NucleoLink™ Strips (Product Guide) Nalge Nunc Video "Why NNI?"
Publications
Detection of immobilized amplicons by ELISA-like techniques (Oroskar,A.A. et al.) TechNote, Vol. 3, No. 16: Quality Control of NucleoLink Strips TechNote, Vol. 3, No. 17: NucleoLink versus CovaLink surfaces TechNote, Vol. 3, No. 18: Thermal Profiles of Liquid in NucleoLink and TopYield Strips TechNote, Vol. 3, No. 19: NucleoLink and TopYield Strips as traditional amplification tubes in commercial thermal cyclers

*The Polymerase Chain Reaction (PCR) process is covered by US patents owned by Hoffmann-La Roche, Inc and F. Hoffmann-La Roche Ltd.